molecular genetic analyses

NimbleGen Sequence Capture

Next-generation sequencing technologies like the 454 Genome Sequencer (Roche) or the Genome Analyzer (Illumina) represent a quantum leap in sequencing technology. Nevertheless, sequencing complete genomes is still very expensive and time-consuming, and many scientific problems focus on certain genomic regions only. Nimblegen Sequence Capture Arrays offer a unique opportunity to specifically enrich your regions of interest prior to sequencing: long oligos (55-100mers) complementary to these genomic regions are directly synthesized on an array and will catch their targets with high specificity and efficiency from a genomic DNA sample in a simple hybridization reaction.

  • 385K and 2,1M feature arrays are available, covering up to 5 MB (385K) or 30 MB (2,1M) of genomic sequence.
  • A proprietary algorithm ensures high specificity of capture oligos, thereby minimizing bias in sequence enrichment.
  • Production of custom arrays is made fast and easy by NimbleGen´s maskless array synthesizer (MAS) technology.
  • Enrichment level can be rapidly assessed by built-in control oligos.

NimbleGen-based Services

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