Array-based Comparative Genomic Hybridization
Introduction
The Agilent microarray platform is among the world’s leading DNA chip technologies. Based on 60mer oligonucleotides and a very flexible ink jet printing device, Agilent´s Comparative Genomic Hybridization (CGH) arrays provide a powerful tool for the genome-wide detection of copy number variations (CNVs). The resolution of array-based CGH is up to 150 times better in comparison to conventional chromosome-based CGH. Previously unknown submicroscopic chromosomal imbalances in patients with cancer, mental retardation, congenital anomalies and developmental disorders can be detected.
Agilent oligo-CGH arrays offer many outstanding features:
- High reproducibility (CV of signal values <10%)
- Cost-effective multiplex formats (2plex, 4plex, 8plex)
- Low amount of starting material needed (500 ng of genomic DNA)
Agilent CGH arrays are available for man, mouse and rat. In addition, the production of custom chips is fast, easy, and cost-effective. Customers may order just a single custom array allowing them to precisely zoom into chromosomal regions of interest.
Applications
Detection of chromosomal copy number variations:- Whole chromosome gains/losses
- Microdeletions
- Duplications
- Unbalanced subtelomeric rearrangements
Service range
- Full Service: customer sends genomic test and reference DNA (1.5 µg each)
- Quality and quantity control of genomic DNA
- Restriction digestion
- Labeling
- Purification of labeled DNA
- Prehybridisation steps
- Hybridization, washing, and scanning of appropriate Agilent microarray
- Determination of CNVs with Agilent´s DNA Analytics software
- Core Service: customer sends labelled and purified DNA (3-6 µg), Core Service comprises steps five to seven of Full Service.
- Additional Service (extra costs apply): Array Design with Agilent´s eArray Tool
Quality control
- Quality and quantity control of genomic DNA by gel analysis and by photometric analysis.
- Quantity and Cy3/Cy5 incorporation rate of each Cy-labelled DNA sample synthesized at ATLAS Biolabs will be determined by photometric analysis.
- All quality assurance procedures are electronically documented and available for our customers on demand.
Data delivery
- 2–4 weeks after receipt of samples (for >30 samples, inquire)
- CD-ROM/DVD sent to customer including:
- Original data
- Results file in EXCEL or TXT format
ISO 9001 service at ATLAS Biolabs is always oriented along internal quality rules which guarantee a constant quality of our work.
