Sequenom iPlex SNP Genotyping Service

The iPlex Gold Genotyping Assay from Sequenom applies robust single-base primer extension to discriminate between the two alleles of a polymorphic DNA site. The main advantages of this assay are:

• Very fast assay design: a new assay can be designed within a few hours. In addition, a semi-automated workflow and unmodified oligos provide rapid turnaround times.
• Highly precise and quantitative detection.
• High degree of multiplexing: up to 40plex.
• High SNP conversion rates: >95%.
• Only 10 ng DNA are needed per 40plex reaction.
• Assay works well with degraded DNA from formalin-fixed paraffin embedded (FFPE) tissue.

• ATLAS Biolabs’ team disposes of longstanding experience with various SNP genotyping technologies.
• Our service is fast (projects can be completed within 3 weeks) and extremely reliable.
• ATLAS Biolabs is certified according to the international standards ISO 9001:2008 and ISO 13485.

• Assay design based on customer´s SNP list.
• Customer sends DNA, lyophilized, in plate format, 10 nanograms per well.
• Sample processing: locus-specific PCR, single-base primer extension, chip spotting, mass spectrometry.
• Data analysis with Typer™ software (Sequenom).

ATLAS Biolabs is certified according to the international standards ISO 9001:2008 and ISO 13485. Our processes are subject to rigorous quality control at all steps.

• ATLAS Biolabs runs a positive and a negative control in each sample plate.
• All quality assurance procedures are electronically documented and available for our customers on demand.