RainDance Sequence Enrichment Service

General Information

RainDance Droplet Technology (RDT), invented by RainDance Technologies, combines the simplicity, specificity and robustness of polymerase chain reaction (PCR) with a highly innovative microfluidic device. A library of up to 20,000 primer pairs is produced by generating microdroplets containing up to five primer pairs, which are subsequently pooled into a single droplet library emulsion. The primer pair droplets are then fused with droplets containing the genomic DNA template plus PCR reagents. One million microdroplets are produced in a single run, with each droplet serving as a reaction vessel for up to five PCR reactions. A maximum of 24 Mb of genomic sequence can be specifically enriched starting from just 3 micrograms of genomic DNA with very low sequence-generated bias.

Advantages of RainDance Droplet Technology

Very high percentage of target bases covered by the design:
up to 100 %
Very high percentage of target amplicons covered by
sequencing: ≥ 95 %
Excellent uniformity of coverage: > 80 % of targets have less than 5-fold difference in target representation

Predesigned Research Screening Panels

Raindance has designed several optimized panels for highly competitive areas of research in collaboration with leading academic centers:

The XSeq panel was designed in cooperation with Emory University, it comprises more than 800 genes and is the most comprehensive genetic screening panel for the human X chromosome.
The OncoSeq panel covers more than 99% of 142 oncogenes and detects many different mutation types, e.g. missense and nonsense mutations, amplifications, frameshifts, splice site mutations, and deletions.
The HLASeq panel interrogates the complete HLA super locus (3.8 Mb region) and covers not just exons, but also introns in this complex region.
The ASDSeq panel was designed in cooperation with Emory University and Greenwood Genetic Center. It covers 25 X-chromosome genes and 37 autosomal genes.

	XSeq	OncoSeq	HLASeq	ASDSeq	ADMESeq (Core)
no. of genes	>800	142	>200	62	36
no. of amplicons	11,575	3,999	3,535	2,349
coverage of target regions	>98%	>99%	>99%	>99%	100%
non-coding sequences covered	5´ promotor & 3´ UTR	5´ promotor & 3´ UTR	introns	5´ promotor & 3´ UTR

Service Range

RainDance Sequence Enrichment Service is available for batches of 8/16/24 etc. samples.
ATLAS Biolabs covers the complete process from library design to the generation of enriched samples. The customer just needs to send genomic coordinates, RefSeq IDs, or Gene IDs, in order to start the design process. Design and library generation are adapted to the sequencing platform preferred by the customer.
If requested, ATLAS Biolabs will also perform next-generation sequencing of the enriched sample.

Quality Control

ATLAS Biolabs is certified according to the international standards ISO 9001:2008 and ISO 13485. Our production processes are subject to rigorous quality control at all steps:

Quality and quantity of each DNA sample sent to ATLAS Biolabs will be determined by agarose gel analysis and by photometric analysis. Samples which do not pass QC will not be processed.
Numerous control steps are executed during library preparation, e. g. QC of successful DNA fragmentation, QC of successful PCR reaction, etc.
All quality assurance procedures are electronically documented and available for our customers on demand.

Sample Delivery

The customer will receive a sufficient amount of enriched DNA to perform next-generation sequencing on his platform of choice.
The customer will be provided with a documentation of successful enrichment.

Timeline

Library design: 1 week
Production of PCR primer library: 6 weeks. Sequence enrichment: 2–3 weeks

Related ATLAS Biolabs Service

Next-Generation Sequencing Service